![]() ![]() Jenkins is available to help new users on the design, implementation, and analysis of DLS data on a fee-per-service basis. The volume of sample needed depends on the plate used (see above) and the number of wells used (duplicate samples are recommended). We recommend starting with 0.5 - 1 mg/mL and trying multiple concentrations. Prepare the sample by adding 5 L of sample to 95 L of Milli-Q water. DLS measures the hydrodynamic radius (RH) of a molecule ( Fig. Smaller particles will have rapid movements, and larger particles will have slower motion. as low as 0.125 mg/mL can be used whereas a protein with a molecular weight of ˜120 kDa could be used at a conc. Dynamic light scattering (DLS) works on the principle of Brownian movement. Using lysozyme (14 kDa) as an example a sample conc. The minimum concentration of the material under investigation depends on the molecular weight, the larger the polymer the lower the conc. The latest DYNAMICS software is available to collect, display, and analyze your data Onboard camera allows user to capture images of each well so images can be inspected for precipitant, bubbles, etc., which can lead to poor results Temperature controlled operation allows for the determination of polydispersity, size, and thermal stability between the range of 4- 85☌ Plate reader accepts 96, 384, 1536-well glass bottom plates with approximate minimum sample volumes of 100, 50, and 5 µL respectively (Average read time for a full 384-well plate is <1 hour) ![]() Quasi-elastic Light Scattering) data which can be used to determine the hydrodynamic radius (size) and size distribution of polymers and biopolymers in solution.ĭetermine the hydrodynamic radius (Rh) and sample polydispersity (Survey multiple buffer conditions to find the buffer that gives the lowest %polydispersity)ĭetermine the Critical Micelle Concentration (CMC) of surfactants under a variety of sample conditions (pH, temp, etc.) We primarily procured this instrument because in addition to being a very sensitive DLS instrument, it could also operate with the smallest sample volume: approximately 10 microliters. Wyatt technology’s DynaPro Plate Reader II instrument allows for high-throughput data collection of Dynamic Light Scattering (DLS, a.k.a. The DynaPro Titan DLS instrument was bought in our department primarily for studying the multimeric status of different proteins. ![]()
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